黄河

个人信息：

黄河 博士

研究员，国科大杭州高等研究院

浙江省杭州市西湖区象山支路1号 310012                            

Phone: 0571-86080119

E-mail:hhuang@ucas.ac.cn

教育背景：

1. 2004.09–2010.07, 中国科学院上海药物研究所, 药物设计学, 博士

2. 2000.09–2004.07, 同济大学, 应用化学, 学士

工作经历：

1. 2023.04至今，国科大杭州高等研究院，药学科学与技术学院，研究员、课题组长

2. 2019.01至今，中国科学院上海药物研究所，化学生物学研究中心，研究员、课题组长

3. 2015.09–2019.01，美国芝加哥大学，肿瘤研究系，研究助理

4. 2012.11–2015.08，美国芝加哥大学，肿瘤研究系，博士后

5. 2010.08–2012.10，美国西北大学，化学系，博士后

研究方向：

工作聚焦于蛋白质新型翻译后修饰研究，一方面发现蛋白新型翻译后修饰及其关键调控因子，并结合临床资源和数据揭示其在疾病演进过程中的作用机制；另一方面，鉴定代谢调节活性天然产物分子的作用靶标，阐释其通过翻译后修饰介导表型的新机制。

代表性研究成果：

1. 1.Song X^#, Ren X^#, Mei Q, Liu H*, Huang H*. Advancing In-Depth N-Terminomics Detection with a Cleavable 2-Pyridinecarboxyaldehyde Probe. J Am Chem Soc, 2024, DOI, 10.1021/jacs.4c02222.

2. 2.Li J^#, Lu L^#, Liu L^#, Ren X, Chen J, Yin X, Xiao Y, Li J, Wei G, Huang H*, Wei W*, Wong J*. HDAC1/2/3 Are Major Histone Desuccinylase Critical for Promoter Desuccinylation. Cell Discov, 2023, 9, 85.

3. 3.Gao Y^#, Sheng X^#, Tan D^#, Kim S^#, Choi S, Paudel S, Lee T, Yan C, Tan M, Kim KM, Cho SS, Ki SH, Huang H*, Zhao Y*, Lee SK*. Identification of Histone Lysine Acetoacetylation as a Dynamic Post‐Translational Modification Regulated by HBO1. Adv Sci, 2023, 2300032.

4. 4.Yang Z^#, Yan C^#, Ma J^#, Peng P^#, Ren X, Cai S, Shen X, Wu Y, Zhang S, Wang X, Qiu S, Zhou J, Fan J, Huang H*, Gao Q*. Lactylome suggests novel mechanisms of metabolic adaptation in hepatocellular carcinoma. Nat Metab., 2023, 5, 61.

5. 5.Tan D, Wei W, Han Z, Ren X, Yan C, Qi S, Song X, Zheng YG, Wong J, Huang H*. HBO1 Catalyzes Lysine Benzoylation in Mammalian Cells. iSCIENCE, 2022, 25, 105443.

6. 6.Wang J^#, Zhao J^#, Yan C^#, Xi C^#, Wu C, Zhao J, Li F, Ding Y, Zhang R, Qi S, Li X, Liu C, Hou W, Chen H, Wang Y, Wu D, Chen K, Jiang H, Huang H*, Liu H*. Identification and evaluation of a lipid-lowering small compound in preclinical models and in a Phase I trial. Cell Metab, 2022, 34, 667.

7. 7.Zeng Y, Shi W, Dong Q, Li W, Zhang J, Ren X, Tang C, Liu B, Song Y, Wu Y, Diao X, Zhou H, Huang H, Tang F, Huang W*. A Traceless Site-Specific Conjugation on Native Antibodies Enables Efficient One-Step Payload Assembly. Angew Chem Int Ed, 2022, 61, e202204132.

8. 8.Huang H^#,*, Zhang D ^#, Weng Y, Delaney K, Tang Z, Yan C, Qi S, Peng C, Cole PA, Roeder RG, Zhao Y^*. The regulatory enzymes and protein substrates for the lysine β-hydroxybutyrylation pathway. Sci Adv, 2021, 7, eabe2771.

9. 9.Koronowski K^#,*, Greco C^#,*, Huang H, Kim J, Fribourgh J, Crosby P, Mathur L, Ren X, Partch C, Jang C, Qiao F, Zhao Y, Sassone-Corsi P. Ketogenesis impact on liver metabolism revealed by proteomics of lysine β-hydroxybutyrylation. Cell Rep, 2021, 36, 109487.

10. 10.Shi W^#, Tang F^#, Ao J, Yu Q, Liu J, Tang Y, Jiang B, Ren X, Huang H, Yang W^*, Huang W^*. Manipulating the Click Reactivity of Dibenzoazacyclooctynes: From Azide Click Component to Caged Acylation Reagent by Silver Catalysis. Angew Chem Int Ed, 2020, 59, 19940.

11. 11.Zhang D^#, Tang Z^#, Huang H, Zhou G, Cui C, Weng Y, Liu W, Kim S, Lee S, Perez-Neut M, Ding J, Czyz D, Hu R, Ye Z, He M, Zheng YG, Shuman HA, Dai L, Ren B, Roeder RG, Becker L^*, Zhao Y^*. Metabolic regulation of gene expression by histone lactylation. Nature, 2019, 574, 575.

12. 12.Huang H^#, Tang S^#, Ji M^#, Tang Z, Shimada M, Liu X, Qi S, Locasale JW, Roeder RG, Zhao Y^*, Li X^*. p300-Mediated Lysine 2-Hydroxyisobutyrylation Regulates Glycolysis. Mol Cell, 2018, 70, 663.

13. 13.Huang H^#, Zhang D, Wang Y, Perez-Neut M, Han Z, Zheng YG, Hao Q, Zhao Y^*. Lysine benzoylation is a histone mark regulated by SIRT2. Nat Commun, 2018, 9, 3374.

14. 14.Huang H^#, Luo Z^#, Qi S^#, Huang J^#, Xu, P, Wang X, Gao L, Li F, Wang J, Zhao W, Gu W, Chen Z, Dai L^*, Dai J^*, Zhao Y^*. Landscape of the Regulatory Elements for Lysine 2-Hydroxyisobutyrylation Pathway. Cell Res, 2018, 28, 111.

15. 15.Kaczmarska Z^#, Ortega E^#, Goudarzi A, Huang H, Kim S, Márquez JA, Zhao Y, Khochbin S^*, Panne D^*. Structure of p300 in complex with acyl-CoA variants. Nat. Chem. Biol., 2017, 13, 21.

16. 16.Huang J^#, Luo Z^#, Ying W^#, Cao Q, Huang H, Dong J, Wu Q, Zhao Y, Qian X^*, Dai J^*. 2-Hydroxyisobutyrylation on histone H4K8 is regulated by glucose homeostasis in Saccharomyces cerevisiae. Proc. Natl. Acad. Sci. U. S. A., 2017, 114, 8782.

17. 17.Xie Z^#, Zhang D^#, Chung D^#, Tang Z, Huang H, Dai L, Qi S, Li J, Colak G, Chen Y, Xia C, Peng C, Ruan H, Kirkey M, Tan M, Lombard DB, White K, Zhao H, Li J, Roeder RG, Yang X^*, Zhao Y^*. Metabolic Regulation of Gene Expression by Histone Lysine β-Hydroxybutyrylation. Mol. Cell, 2016, 62, 194.

18. 18.Li Y^#, Sabari BR^#, Panchenko T^#, Wen H, Zhao D, Guan H, Wan L, Huang H, Tang Z, Zhao Y, Roeder RG, Shi X, Allis CD^*, Li H^*. Molecular Coupling of Histone Crotonylation and Active Transcription by AF9 YEATS Domain. Mol. Cell, 2016, 62, 181.

19. 19.Goudarzi A^#, Zhang D^#, Huang H, Barral S, Kwon OK, Qi S, Tang Z, Buchou T, Vitte AL, He T, Cheng Z, Mintellier E, Gaucher J, Curtet S, Debernardi A, Charbonnier J, Curtet S, Debernardi A, Charbonnier G, Puthier D, Petosa C, Panne D, Rousseaux S, Roeder RG, Zhao Y^*, Khochbin S^*. Dynamic Competing Histone H4 K5K8 Acetylation and Butyrylation Are Hallmarks of Highly Active Gene Promoters. Mol. Cell, 2016, 62, 169.

20. 20.Martínez-Reyes I^#, Diebold LP, Kong H, Schieber M, Huang H, Hensley CT, Mehta MM, Wang T, Santos JH, Dufour E, Spelbrink JN, Weinberg SE, Zhao Y, DeBerardinis RJ, Chandel NS^*. TCA Cycle and Mitochondrial Membrane Potential Are Necessary for Diverse Biological Functions. Mol. Cell, 2016, 61, 199.

21. 21.Huang H^#, Lin S, Garcia BA, Zhao Y^*. Quantitative Proteomic Analysis of Histone Modifications, Chem. Rev., 2015, 115, 2376.

22. 22.Sabari BR^#, Tang Z, Huang H, Yong-Gonzalez V, Molina H, Kong HE, Dai L, Shimada M, Cross JR, Zhao Y, Roeder RG, Allis CD^*. Intracellular Crotonyl-CoA Stimulates Transcription through p300-Catalyzed Histone Crotonylation, Mol. Cell, 2015, 58, 203.

23. 23.Huang H^#, Sabari BR, Garcia BA, Allis CD, Zhao Y^*. SnapShot: Histone Modifications. Cell, 2014, 159, 458.

24. 24.Tan M^#, Peng C^#, Anderson KA^#, Chhoy P, Xie Z, Dai L, Park J, Chen Y, Huang H, Zhang Y, Ro J, Wagner GR, Green MF, Madsen AS, Schmiesing J, Peterson BS, Xu G, Ilkayeva OR, Muehlbauer MJ, Braulke T, Mühlhausen C, Backos DS, Olsen CA, McGuire PJ, Pletcher SD, Lombard DB, Hirschey MD^*, Zhao Y^*. Lysine Glutarylation Is a Protein Posttranslational Modification Regulated by SIRT5. Cell Metab., 2014, 19, 605.

25. 25.Huang H^#, Ji H, Li H, Jing Q, Labby KJ, Martásek P, Roman LJ, Poulos TL^*, Silverman RB^*. Selective monocationic inhibitors of neuronal nitric oxide synthase. Binding mode insights from molecular dynamics simulations. J Am Chem Soc, 2012, 134, 11559.

26. 26.Ba Q^#, Hao M^#, Huang H^#, Hou J, Ge S, Zhang Z, Yin J, Chu R, Jiang H, Wang F, Chen K, Liu H^*, Wang H^*. Iron deprivation caused by a novel iron chelator thiosemicarbazone-24 suppresses hepatocellular carcinoma in vitro and in vivo. Clin Cancer Res, 2011, 17, 7625.