黄河

个人信息：

黄河 博士

研究员，国科大杭州高等研究院

浙江省杭州市西湖区象山支路1号 310012

Phone: 0571-86080119

E-mail:hhuang@ucas.ac.cn

教育背景：

1. 2004.09–2010.07, 中国科学院上海药物研究所, 药物设计学, 博士

2. 2000.09–2004.07, 同济大学, 应用化学, 学士

工作经历：

1. 2023.04至今，国科大杭州高等研究院，药学科学与技术学院，研究员、课题组长

2. 2019.01至今，中国科学院上海药物研究所，化学生物学研究中心，研究员、课题组长

3. 2015.09–2019.01，美国芝加哥大学，肿瘤研究系，研究助理

4. 2012.11–2015.08，美国芝加哥大学，肿瘤研究系，博士后

5. 2010.08–2012.10，美国西北大学，化学系，博士后

研究方向：

工作聚焦于蛋白质新型翻译后修饰研究，一方面发现蛋白新型翻译后修饰及其关键调控因子，并结合临床资源和数据揭示其在疾病演进过程中的作用机制；另一方面，鉴定代谢调节活性天然产物分子的作用靶标，阐释其通过翻译后修饰介导表型的新机制。近五年以通讯作者（含共同）在Cell Metab（2篇）、Nat Metab（2篇）、Sci Adv（2篇）、J Am Chem Soc（2篇）等杂志上发表论文60余篇，论文共被引用15000余次。

代表性研究成果：

1. Song X^#, Peng P^#, Zheng H, Zheng Y, Wang Q, Ge Y, Tan D, Chen L, Wang X, Huang H*. Lysine pyruvylation couples glycolytic flux to epigenetic regulation. Nat Metab, 2026, Accepted.

2. Song X, Lu Y, Guo X, Zheng Y, Huang H*. Covalent capture and genetic code expansion enables chemoproteomic profiling and functional characterization of lysine acetoacetylation. Sci Adv, 2026, 12, eaeb5106.

3. Wang J^#, Wang H^#, Du R^#, Li C^#, Su M, Wang S, Kan W, Liu G, Zhang Y, Hu X, Gao F, Xu G, Li C, Zhu W, Ye Y, Sheng L, Shi Y, Shao Y, Song J, Wang Y, Wang B, Zhou Y*, Huang H*, Li J*, Liu H*. Potent and selective LSD1 inhibitor DC551040 reveals a promising combination therapy for AML with insight into epigenetic dysregulation. Signal Transduct Target Ther, 2026, 11, 108.

4. Liu R, Ren X, Park YE, Feng H, Sheng X, Song X, AminiTabrizi R, Shah H, Li L, Zhang Y, Abdullah KG, Dubois-Coyne S, Lin H, Cole PA, DeBerardinis RJ, McBrayer SK, Huang H*, Zhao Y*. Nuclear GTPSCS functions as a lactyl-CoA synthetase to promote histone lactylation and gliomagenesis. Cell Metab, 2025, 37, 377.

5. Liu G^#, Chen L^#, Zhao J, Jiang Y, Guo Y, Mao X, Ren X, Liu K, Mei Q*, Li Q*, Huang H*. Deciphering the Metabolic Impact and Clinical Relevance of N-Glycosylation in Colorectal Cancer through Comprehensive Glycoproteomic Profiling. Adv Sci, 2025, 2415645.

6. Lyu X^#, Wang X^#, Lin D^#, Lu Y^#, Wang C, Yan Z, Wang Z, Cheng Y, Cheng J, Ren X, Su Y, Zhang S, Chen Y*, Huang H*, Zhao Y*. Synthesis of an RBM39 Degrader That Downregulates CEP192 and Induces Disorganized Spindle Structures. J Med Chem, 2025, 68, 7807.

7. Song X^#, Ren X^#, Mei Qi, Liu H*, Huang H*. Advancing In-Depth N-Terminomics Detection with a Cleavable 2-Pyridinecarboxyaldehyde Probe. J Am Chem Soc, 2024, 146, 6487.

8. Lin Z^#, Liu B^#,*, Lu M, Wang Y, Ren X, Liu Z, Luo C, Shi W, Zou X, Song X, Tang F, Huang H*, Huang W*. Controlled Reversible N-Terminal Modification of Peptides and Proteins. J Am Chem Soc, 2024, 146, 23752.

9. Yang Z^#, Yan C^#, Ma J^#, Peng P^#, Ren X, Cai S, Shen X, Wu Y, Zhang S, Wang X, Qiu S, Zhou J, Fan J, Huang H*, Gao Q*. Lactylome suggests novel mechanisms of metabolic adaptation in hepatocellular carcinoma. Nat Metab, 2023, 5, 61.

10. Gao Y^#, Sheng X^#, Tan D^#, Kim S^#, Choi S, Paudel S, Lee T, Yan C, Tan M, Kim KM, Cho SS, Ki SH, Huang H*, Zhao Y*, Lee SK*. Identification of Histone Lysine Acetoacetylation as a Dynamic Post‐Translational Modification Regulated by HBO1. Adv Sci, 2023, 2300032.

11. Wang J^#, Zhao J^#, Yan C^#, Xi C^#, Wu C, Zhao J, Li F, Ding Y, Zhang R, Qi S, Li X, Liu C, Hou W, Chen H, Wang Y, Wu D, Chen K, Jiang H, Huang H*, Liu H*. Identification and evaluation of a lipid-lowering small compound in preclinical models and in a Phase I trial. Cell Metab., 2022, 667.

12. Zeng Y, Shi W, Dong Q, Li W, Zhang J, Ren X, Tang C, Liu B, Song Y, Wu Y, Diao X, Zhou H, Huang H, Tang F, Huang W*. A Traceless Site-Specific Conjugation on Native Antibodies Enables Efficient One-Step Payload Assembly. Angew Chem Int Ed, 2022, 61, e202204132.

13. Huang H^#,*, Zhang D ^#, Weng Y, Delaney K, Tang Z, Yan C, Qi S, Peng C, Cole PA, Roeder RG, Zhao Y^*. The regulatory enzymes and protein substrates for the lysine β-hydroxybutyrylation pathway. Sci. Adv., 2021, 7, eabe2771.

14. Shi W^#, Tang F^#, Ao J, Yu Q, Liu J, Tang Y, Jiang B, Ren X, Huang H, Yang W^*, Huang W^*. Manipulating the Click Reactivity of Dibenzoazacyclooctynes: From Azide Click Component to Caged Acylation Reagent by Silver Catalysis. Angew Chem Int Ed, 2020, 59, 19940.

15. Di Zhang^#, Zhanyun Tang^#, He Huang, Guolin Zhou, Chang Cui, Yejing Weng, Wenchao Liu, Sunjoo Kim, Sangkyu Lee, Mathew Perez-Neut, Jun Ding, Daniel Czyz, Rong Hu, Zhen Ye, Maomao He, Y George Zheng, Howard A Shuman, Lunzhi Dai, Bing Ren, Robert G Roeder, Lev Becker*, Yingming Zhao*. Metabolic regulation of gene expression by histone lactylation. Nature 2019, 574, 575.

16. He Huang^#, Shuang Tang^#, Ming Ji^#, Zhanyun Tang, Miho Shimada, Xiaojing Liu, Shankang Qi, Jason W Locasale, Robert G Roeder, Yingming Zhao*, Xiaoling Li*. EP300-Mediated Lysine 2-Hydroxyisobutyrylation Regulates Glycolysis. Mol. Cell 2018, 70, 663-678.

17. He Huang^#, Di Zhang, Yi Wang, Mathew Perez-Neut, Zhen Han, Y. George Zheng, Quan Hao, Yingming Zhao*. Lysine benzoylation is a histone mark regulated by SIRT2. Nat. Commun. 2018, 9, 3374.

18. Huang H^#, Luo Z^#, Qi S^#, Huang J^#, Xu, P, Wang X, Gao L, Li F, Wang J, Zhao W, Gu W, Chen Z, Dai L^*, Dai J^*, Zhao Y^*. Landscape of the Regulatory Elements for Lysine 2-Hydroxyisobutyrylation Pathway. Cell Res, 2018, 28, 111.

19. Kaczmarska Z^#, Ortega E^#, Goudarzi A, Huang H, Kim S, Márquez JA, Zhao Y, Khochbin S^*, Panne D^*. Structure of p300 in complex with acyl-CoA variants. Nat Chem Biol, 2017, 13, 21.

20. Huang J^#, Luo Z^#, Ying W^#, Cao Q, Huang H, Dong J, Wu Q, Zhao Y, Qian X^*, Dai J^*. 2-Hydroxyisobutyrylation on histone H4K8 is regulated by glucose homeostasis in Saccharomyces cerevisiae. Proc Natl Acad Sci USA, 2017, 114, 8782.

21. Xie Z^#, Zhang D^#, Chung D^#, Tang Z, Huang H, Dai L, Qi S, Li J, Colak G, Chen Y, Xia C, Peng C, Ruan H, Kirkey M, Tan M, Lombard DB, White K, Zhao H, Li J, Roeder RG, Yang X^*, Zhao Y^*. Metabolic Regulation of Gene Expression by Histone Lysine β-Hydroxybutyrylation. Mol Cell, 2016, 62, 194.

22. Li Y^#, Sabari BR^#, Panchenko T^#, Wen H, Zhao D, Guan H, Wan L, Huang H, Tang Z, Zhao Y, Roeder RG, Shi X, Allis CD^*, Li H^*. Molecular Coupling of Histone Crotonylation and Active Transcription by AF9 YEATS Domain. Mol Cell, 2016, 62, 181.

23. Goudarzi A^#, Zhang D^#, Huang H, Barral S, Kwon OK, Qi S, Tang Z, Buchou T, Vitte AL, He T, Cheng Z, Mintellier E, Gaucher J, Curtet S, Debernardi A, Charbonnier J, Curtet S, Debernardi A, Charbonnier G, Puthier D, Petosa C, Panne D, Rousseaux S, Roeder RG, Zhao Y^*, Khochbin S^*. Dynamic Competing Histone H4 K5K8 Acetylation and Butyrylation Are Hallmarks of Highly Active Gene Promoters. Mol Cell, 2016, 62, 169.

24. Martínez-Reyes I^#, Diebold LP, Kong H, Schieber M, Huang H, Hensley CT, Mehta MM, Wang T, Santos JH, Dufour E, Spelbrink JN, Weinberg SE, Zhao Y, DeBerardinis RJ, Chandel NS^*. TCA Cycle and Mitochondrial Membrane Potential Are Necessary for Diverse Biological Functions. Mol Cell, 2016, 61, 199.

25. He Huang^#, Shu Lin, Benjamin A Garcia, Yingming Zhao*. Quantitative proteomic analysis of histone modifications. Chem. Rev. 2015, 115, 2376-2418.

26. Sabari BR^#, Tang Z, Huang H, Yong-Gonzalez V, Molina H, Kong HE, Dai L, Shimada M, Cross JR, Zhao Y, Roeder RG, Allis CD^*. Intracellular Crotonyl-CoA Stimulates Transcription through p300-Catalyzed Histone Crotonylation, Mol Cell, 2015, 58, 203.

27. He Huang^#, Benjamin R Sabari, Benjamin A Garcia, C David Allis, Yingming Zhao*. SnapShot: histone modifications. Cell 2014, 159, 458-458.

28. Tan M^#, Peng C^#, Anderson KA^#, Chhoy P, Xie Z, Dai L, Park J, Chen Y, Huang H, Zhang Y, Ro J, Wagner GR, Green MF, Madsen AS, Schmiesing J, Peterson BS, Xu G, Ilkayeva OR, Muehlbauer MJ, Braulke T, Mühlhausen C, Backos DS, Olsen CA, McGuire PJ, Pletcher SD, Lombard DB, Hirschey MD^*, Zhao Y^*. Lysine Glutarylation Is a Protein Posttranslational Modification Regulated by SIRT5. Cell Metab, 2014, 19, 605.

29. Huang H^#, Li H, Yang S, Chreifi G, Martásek P, Roman LJ, Meyskens FL, Poulos TL^*, Silverman RB^*. Potent and Selective Double-headed Thiophene-2-carboximidamide Inhibitors of Neuronal Nitric Oxide Synthase for the Treatment of Melanoma. J Med Chem., 2014, 57, 686.

30. Huang H^#, Li H, Martásek P, Roman LJ, Poulos TL^*, Silverman RB^*. Structure-Guided Design of Selective Inhibitors of Neuronal Nitric Oxide Synthase. J Med Chem, 2013, 56, 3024.

31. Huang H^#, Ji H, Li H, Jing Q, Labby KJ, Martásek P, Roman LJ, Poulos TL^*, Silverman RB^*. Selective monocationic inhibitors of neuronal nitric oxide synthase. Binding mode insights from molecular dynamics simulations. J Am Chem Soc, 2012, 134, 11559.

32. Huang H^#, Chen Q^#, Ku X, Meng L, Lin L, Wang X, Zhu C, Wang Y, Chen Z, Li M, Jiang H, Chen K, Ding J, Liu H^*. A series of α-heterocyclic carboxaldehyde thiosemicarbazones inhibit topoisomerase IIα catalytic activity, J Med Chem, 2010, 53, 3048.